Infectious bursal disease virus ( ibdv ) , the causative agent of infectious bursal disease ( ibd ) , causes immunosuppression in young chickens by destroying the precursors of b lymphocytes in the bursa of fabricius 传染性法氏囊病( ibd )是由传染性法氏囊病病毒( ibdv )引起的急性接触性传染病，是危害世界养禽业的主要传染病之一。
3.
The chickens and chicken embryos were inoculated with variant serotype isolate e of infectious bursal disease virus using cloacal , nosal routes or via the allantoic cavity route , and the histopathological features of the bursa of fabricius of the ibdv _ infected chickens at various intervals of time were systematically investigated 本试验全面而系统地观察了传染性法氏囊病病毒变异e株，通过泄殖腔、鼻腔和尿囊腔接种雏鸡和鸡胚后不同时间法氏囊的组织形态学变化。
4.
Three ibdv strains , designated as zj2000 , zj991 and zj992 , were isolated from bursa of fabricius of the sick chicken flocks in hangzhou , shenzhou , and ningbo of zhejiang province . the isolates could react with ibdv positive reference serum in the agar immune - diffusion test and caused 30 - 100 % mortality when passaged in chicken embryos . chickens inoculated with these isolates exhibited clinical and pathological signs typical of infectious bursal disease 从浙江省杭州、嵊州，宁波等地暴发疑似传染性法氏囊病的鸡群中采集法氏囊病料(编号分别为zj2000 、 zj991 、 zj992 ) ，分离病毒，经琼脂免疫扩散试验、动物回归试验、鸡胚回归试验和电镜观察等证实所分离的3株野毒是传染性法氏囊病病毒。
5.
Spf chickens with 21 - day - old were infected subcutaneously with oil - emulsion vaccine of ibdv of germinal or cellcular and injected intramuscularly with different dosages bursin which gain through ultrafilter . lt is proved that bursin of chickens and ducks can both shorten the time of antibody induced against ibdv , raise the level of serum antibody . they make chickens obtaining strong immunocompetente in a short time . agp liters of the group of infecting 0 . 4mlcbs + ibdv of germinal and 0 . 8mlcbs + ibdv of cellcular or 0 . 8mldbs + ibdv of germinal and cellcular are higher than immune control group about 2 liters averagely . the chickens were inoculated with ibdv live vaccine mixed with the different dosages of lyophilized bursin by the eye drop method . the results sugest that cbs or dbs of different dosages can both improve the antibody inducation to different age chickens against ibdv . they may also alleviate the immunological injury of activated virus to bursa of fabricius . and promote the repairation of the lesion . it can be found that bs can raise body weight gain and feed coversion ratio 将超滤获得的法氏囊活性肽分别以不同剂量肌肉注射21日龄spf鸡，同时颈部皮下注射ibd胚毒或细胞毒灭活苗，结果表明：鸡、鸭法氏囊活性肽都能够缩短ibd油苗诱导产生抗体的时间，提高抗体水平，使鸡可以在比较短的时间内获得坚强的免疫。 0 . 4mlcbs胚毒组和0 . 8mlcbs细胞毒组或0 . 8mldbs胚毒和细胞毒组的agp抗体滴度平均比免疫对照组高2个滴度。将法氏囊活性肽与ibd活苗联合免疫鸡，结果表明：不同剂量的cbs和dbs都可以对不同日龄鸡ibd抗体的产生有不同程度的促进作用；还可以减少弱毒对鸡法氏囊组织的损伤，加快其修复。
